Thunchanok Thongsamer, Soydoa Vinitnantharat, Anawat Pinisakul, David Werner, Bundit Tirachulee, Pavinee Pattanachan, Vassanasak Limkhuansuwan
Journal of soil science and plant nutrition · 2026
Kenneth W. Widmer, Soydoa Vinitnantharat, Thunchanok Thongsamer, Pauline Dianne Santos, Suthipong Sthiannopkao
Journal of Materials & Environmental Sustainability Research · 2024
Utilizing molecular methods for monitoring and determining water quality in developing countries can be problematic. Effective extraction and storage of DNA from environmental samples is especially difficult due to lack of access to sufficient laboratory facilities for molecular work. Use of a commercial product for the extraction of DNA, as well as its application in determining the microbial quality of surface waters, was evaluated and compared. Surface water samples collected from the Chao Phraya River in Thailand were processed to enumerate Escherichia coli. Quantification was done through culture methods (Colilert Quanti-tray) and quantitative real-time PCR (SYBR green and Taqman assays). Processing of surface water samples and culture enumeration of E. coli was done in Thailand, while DNA extraction and enumeration using real-time PCR was done in Korea. E. coli MPN counts were mean log 2.89/100ml. Quantitative real-time PCR assays counts ranged from 2.38 to 3.59 mean log CFU/100 ml. Taqman PCR assays had no significant difference in mean log counts compared to conventional culture methods. However mean log counts of SYBR green PCR assays were significantly higher than counts obtained by culture methods and Taqman PCR assays (P<0.05). Results demonstrated FTA Elute cards can be employed for the extraction of microbial DNA from surface waters and have potential use in detection of fecal indicator bacteria from environmental water samples.
Thunchanok Thongsamer, Soydoa Vinitnantharat, Anawat Pinisakul, David Werner
Sustainable Environment Research · 2022
Abstract The presence of excess nutrients in water resources can be harmful to human health and aquatic ecosystems. To develop an affordable water treatment method, the agricultural waste material coconut husk was converted into a low-cost adsorbent by thermal conversion to biochar, pelletized without (CH), and with chitosan (CHC), or eggshell powder (CHEG) modifications. The physical and chemical properties of all adsorbents were characterized using Brunauer-Emmett-Teller (BET) surface analysis, Fourier transform infrared spectroscopy, scanning electron microscopy, pH zpc , iodine number and elemental analysis. The adsorption of ammonium (NH 4 + ), nitrate (NO 3 − ), and phosphate (PO 4 3− ) in single and mixed solute solutions was investigated for initial concentrations of 10 mg L − 1 . Langmuir, Freundlich, Sips, Dubinin-Radushkevich (D-R) and BET isotherm models were used to investigate the adsorption mechanisms. The maximum adsorption capacity of NH 4 + on CH, CHC, and CHEG from mixed solute solution was 5.0, 4.7 and 5.9 mg g − 1 , respectively, while the adsorption capacity of mixed:single solute solution was 0.95, 0.93, and 1.04, respectively. CH, CHC, and CHEG had greater ability to remove the cation NH 4 + than anions NO 3 − and PO 4 3− from aqueous solution. The highest maximum adsorption capacity for anions NO 3 − and PO 4 3− was found on CHEG (1.7 mg g − 1 ) and CH (6.7 mg g − 1 ), respectively. NH 4 + and NO 3 − were bound by chemisorption as indicated by D-R isotherm E values (> 8 kJ mol − 1 ), and enthalpy ∆H values (> 80 kJ mol − 1 ). In contrast, PO 4 3− adsorption was mainly by physical interaction, including pore-filling, and electrostatic attraction. Pseudo first order and pseudo second order models provided good fits of the sorption kinetics data ( R 2 > 0.9). The initial concentrations of NH 4 + , NO 3 − , and PO 4 3− in surface water sampled from a canal in Bangkok were 10.4, 1.2, and 3.9 mg L − 1 , respectively, which indicated eutrophication. At a dose of 20 g L − 1 , CHC achieved the best nutrient removal from this surface water, by 24% for NH 4 + , 25% for NO 3 − , and 66% for PO 4 3− after 48 h contact, respectively.
David Werner, Kishor Acharya, Adrian Blackburn, Rixia Zan, Jidapa Plaimart, Ben Allen, Shaaban M. Mgana, Shadrack Sabai, Franella Francos Halla, Said Maneno Massawa, Alemseged Tamiru Haile, Andualem Mekonnen Hiruy, Jemila Mohammed, Soydoa Vinitnantharat, Thunchanok Thongsamer, Kalyan Pantha, Cesar Rossas Mota Filho, Bruna Coelho Lopes
Water · 2022
In 2014, Oxford Nanopore Technologies (ONT) introduced an affordable and portable sequencer called MinION. We reviewed emerging applications in water research and assessed progress made with this platform towards ubiquitous genetics. With >99% savings in upfront costs as compared to conventional platforms, the MinION put sequencing capacity into the hands of many researchers and enabled novel applications with diverse remits, including in countries without universal access to safe water and sanitation. However, to realize the MinION’s fabled portability, all the auxiliary equipment items for biomass concentration, genetic material extraction, cleanup, quantification, and sequencing library preparation also need to be lightweight and affordable. Only a few studies demonstrated fully portable workflows by using the MinION onboard a diving vessel, an oceanographic research ship, and at sewage treatment works. Lower nanopore sequencing read accuracy as compared to alternative platforms currently hinders MinION applications beyond research, and inclusion of positive and negative controls should become standard practice. ONT’s EPI2ME platform is a major step towards user-friendly bioinformatics. However, no consensus has yet emerged regarding the most appropriate bioinformatic pipeline, which hinders intercomparison of study results. Processing, storing, and interpreting large data sets remains a major challenge for ubiquitous genetics and democratizing sequencing applications.
Wojciech Mrozik, Babak Minofar, Thunchanok Thongsamer, Nathacha Wiriyaphong, Sasiwimol Khawkomol, Jidapa Plaimart, John Vakros, Hrissi K. Karapanagioti, Soydoa Vinitnantharat, David Werner
Journal of Environmental Management · 2021
In this work, we evaluated the valorisation of agricultural waste materials by transforming coconut husks and shells, corncobs and rice straw into biochar for water treatment in aquaculture. We compared the biochars' suitability for removal of organic micropollutants (acetaminophen, oxytetracycline, tetracycline, enrofloxacin, atrazine, diuron and diclofenac) from surface water needed for aquaculture. The biochars were prepared by three methods ranging from inexpensive drum kilns (200 °C) to pyrolysis with biogasfication (350-750 °C). Overall, antibiotics tetracycline and enrofloxacin were the most strongly sorbed micropollutants, and coconut husk biochar prepared at 750 °C was the best sorbent material. Molecular Dynamics simulations indicated that the major sorption mechanism is via π-π stacking interactions and there is a possibility of multilayer sorption for some of the micropollutants. We observed, a strong impact of ionic strength (salinity), which is an important consideration in coastal aquaculture applications. High salinity decreased the sorption for antibiotics oxytetracycline, tetracycline and enrofloxacin but increased diclofenac, atrazine and diuron sorption. We considered coconut husk biochar produced in drum kilns the most practical option for biochar applications in small-scale coastal aquacultures in South Asia. Pilot trials of canal water filtration at an aquaculture farm revealed that micropollutant sorption by coconut husk biochar under real-world conditions might be 10-500 times less than observed in the laboratory studies. Even so, biochar amendment of sand enhanced the micropollutant retention, which may facilitate subsequent biodegradation and improve the quality of brackish surface water used for food production in coastal aquaculture.
Sasiwimol Khawkomol, Rattikan Neamchan, Thunchanok Thongsamer, Soydoa Vinitnantharat, Boonma Panpradit, Prapa Sohsalam, David Werner, Wojciech Mrozik
Sustainability · 2021
A horizontal drum kiln is a traditional method widely used in Southeast Asian countries for producing biochar. An understanding of temperature conditions in the kiln and its influence on biochar properties is crucial for identifying suitable biochar applications. In this study, four agricultural residues (corncob, coconut husk, coconut shell, and rice straw) were used for drum kiln biochar production. The agricultural residues were turned into biochar within 100–200 min, depending on their structures. The suitability of biochar for briquette fuels was analyzed using proximate, ultimate, and elemental analysis. The biochar’s physical and chemical properties were characterized via bulk density, iodine number, pHpzc, SEM, and FTIR measurements. All biochars had low O/C and H/C ratios and negative charge from both carbonyl and hydroxyl groups. Coconut husk and shell biochar had desirable properties such as high heating value and a high amount of surface functional groups which can interact with nutrients in soil. These biochars are thus suitable for use for a variety of purposes including as biofuels, adsorbents, and as soil amendments.
Thunchanok Thongsamer, Rattikan Neamchan, Adrian Blackburn, Kishor Acharya, Sawannee Sutheeworapong, Bundit Tirachulee, Pavinee Pattanachan, Soydoa Vinitnantharat, Xinyuan Zhou, Jian‐Qiang Su, Yong‐Guan Zhu, David W. Graham, David Werner
Journal of Hazardous Materials · 2021
We assessed antimicrobial resistance (AMR) potential to seven major classes of antibiotics in Central Thailand's coastal aquaculture region using high-throughput qPCR targeting 295 antibiotic resistance genes (ARGs) and mobile genetic elements (MGEs). In parallel, we used MinION next generation sequencing (NGS) of 16S rRNA gene amplicons, qPCR of faecal pollution makers, and conventional plate count methods for the comprehensive characterization of environmental microbiomes. We tested the hypothesis that aquaculture increases environmental AMR by comparing ARGs and MGEs in water and sediment samples from five aquaculture sites and their associated canals. There was no evidence from the ARG and MGE data that aquaculture is a major driver of environmental AMR in Central Thailand. Instead, the highest relative prevalence of resistance traits was found in Hua Krabue canal water influenced by urban pollution from Bangkok at the inland edge of the coastal aquaculture region. The sum of ARGs and MGEs, relative to 16S rRNA genes used as markers for overall bacterial abundance, was between 0.495 ± 0.011 and 0.498 ± 0.013 in Hua Krabue canal water, compared with at most 0.132 ± 0.005 in all the other environmental samples. Corresponding patterns were observed for most faecal pollution markers, which were also elevated in Hua Krabue canal water.
Wojciech Mrozik, Soydoa Vinitnantharat, Thunchanok Thongsamer, Nipapun Pansuk, Pavinee Pattanachan, Parinda Thayanukul, Kishor Acharya, Marcos Quintela‐Baluja, Charles R. E. Hazlerigg, Aidan Robson, Russell J. Davenport, David Werner
The Science of The Total Environment · 2019
Peri-urban aquacultures produce nutritious food in proximity to markets, but poor surface water quality in rapidly expanding megacities threatens their success in emerging economies. Our study compared, for a wide range of parameters, water quality downstream of Bangkok with aquaculture regulations and standards. For parameters not meeting those requirements, we sought to establish whether aquaculture practice or external factors were responsible. We applied conventional and advanced methods, including micropollutant analysis, genetic markers, and 16S rRNA amplicon sequencing, to investigate three family-owned aquacultures spanning extensive, semi-intensive and intensive practices. Canals draining the city of Bangkok did not meet quality standards for water to be used in aquaculture, and were sources for faecal coliforms, Bacteriodes, Prevotella, Human E. coli, tetracycline resistance genes, and nitrogen into the aquaculture ponds. Because of these inputs, aquacultures suffered algae blooms, with and without fertilizer and feed addition to the ponds. The aquacultures were sources of salinity and the herbicide diuron into the canals. Diuron was detectable in shrimp, but not at a level of concern to human health. Given the extent and nature of pollution, peri-urban water policy should prioritize charging for urban wastewater treatment over water fees for small-scale agricultural users. The extensive aquaculture attenuated per year an estimated twenty population equivalents of nitrogen pollution and trillions of faecal coliform bacteria inputs from the canal. Extensive aquacultures could thus contribute to peri-urban blue-green infrastructures providing ecosystem services to the urban population such as flood risk management, food production and water pollution attenuation.
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